Dataset on pyrene-labelled apolipoprotein A-I, model development and fitting to monitor oligomeric species of its lipid-free form

The research contains data for the self-association of pyrene-labelled single Cysteine variants of apolipoprotein A-I (apoA-I). The data report apoA-I variants characterizations by guanidinium hydrochloride denaturing curves, fluorescence emission spectra of titrations on pyrene-labelled apoA-I variants, typical signals of pyrene to environmental characterization (Excimer/Monomer and P-value) for corrected spectra and deconvoluted data, and parameters describing self-association of apoA-I represented as a unique equilibrium. Mathematica script to allow deconvolution spectra is available. All the data are presented in MS Excel and the script in Mathematica Wolfram format.本研究收录了单半胱氨酸残基标记芘（pyrene）的载脂蛋白A-I（apolipoprotein A-I，apoA-I）变体的自缔合相关实验数据。数据涵盖通过盐酸胍（guanidinium hydrochloride）变性曲线表征的apoA-I变体特性、芘标记apoA-I变体滴定实验的荧光发射光谱、经校正与解卷积处理后的光谱与数据中，用于表征芘所处环境的典型信号（激基缔合物/单体比与P值），以及以单一平衡态表征apoA-I自缔合过程的相关参数。本研究提供了用于光谱解卷积的Mathematica脚本文件，所有实验数据均以Microsoft Excel格式存储，脚本文件则采用Wolfram Mathematica格式保存。