Efficacy and mechanisms of water extract of Eucommia ulmoides Oliv. male flowers on skin barrier repair and calming

Zebrafish tail fin image, using Image Pro Plus v.5.1 software to calculate zebrafish tail fin area. In GraphPad Prism v.9.0 software, one-way ANOVA analysis of variance was used for significance testing, and the statistical results were expressed as "mean ± standard error (` x ± s)". P<0.05 indicates that the difference is statistically significant. There are specialized formulas for data processing in PCR, and the Excel spreadsheet has shown the specific calculation method. The first column represents the blank group, the second column represents the model group, and the third column represents the positive control group. The last three columns represent the treatment solution groups of Eucommia ulmoides male flowers at concentrations of 100, 140, and 180 μ g/mL, respectively