Single crystal x-ray diffraction data set for human GABARAP complexed with artificial peptide IM-2

Human GABARAP (gamma-aminobutyric acid receptor-associated protein) belongs to the human autophagy-related protein 8 (hATG8) family which is implicated in various aspects of macroautophagy and other vesicle trafficking pathways. The family consists of six homologues classified into two subfamilies, the GABARAP subfamily and the microtubule-associated protein 1 light chain 3 (MAP1LC3) subfamily containing three proteins each. The human GABARAP protein was co-crystallised with the synthetic, artificial peptide IM-2. The crystal selected for data collection was obtained using PEG 8000 and ammonium sulfate as precipitating agents in a sodium cacodylate buffer. Glycerol was added as cryo-protectant during crystal preparation. Single crystal x-ray diffraction data extending to a diffraction limit of 1.42 Å were obtained via a helical scan on beamline ID30A-3 at the European Synchrotron Radiation Facility (ESRF; Grenoble, France), using an x-ray wavelength of 0.968 Å. The crystal structure will reveal the binding mode of the artificial ligand IM-2 to the human GABARAP protein.