Gene Targets and Pre-validation results for CRISPR Cas-9 editng of hydrogenase genes in Rhodobacter johrii isolate MAY2

This dataset contain gene sequences for the small and large sub-unit of uptake hydrogenase found in Rhodobacter johrii obtained through whoke genome sequencing. Moreover, it contains gRNA and primer sequences to target these genes for manipulation. Additionally, this contains in-vitro cleavage assay results pre-validating the gRNA sequence as effective in cleaving the target genes as well as the ability of the primers to amplify the genes.

本数据集收录了从罗氏菌约翰氏亚种（Rhodobacter johrii）中提取的摄取氢酶的小亚基和大亚基的基因序列，这些序列是通过全基因组测序获得的。此外，数据集中还包含了旨在靶向这些基因以便进行操作的反向录酶（gRNA）和引物序列。另外，该数据集还包含了体外切割实验的结果，这些结果预先验证了gRNA序列在切割目标基因方面的有效性，以及引物扩增基因的能力。