Softness limits CTLs self-killing and is inherited to T-leukemic cells for immune escape
收藏科学数据银行2024-01-14 更新2026-04-23 收录
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资源简介：
Using CRISPR/Cas9-based gene overexpression method, we screened membrane or membrane-related protein molecule(s) by transfecting Jurkat cells with membrane protein gRNA library, followed by perforin treatment and PI staining. The top 10% among PI staining high and the bottom 10% among PI staining negative cells were sorted for next-generation sequencing (NGS) . Using the MAGeCK algorithm or RRA analysis, top sgRNAs were identified in PIh cells . We provide evidence that effector CTLs exhibit the perforin-resistant softness by downregulating cytoskeletal protein filamin A (FLNA)and this FLNA-dependent pathway is usurped by leukemic T cells to evade T cell killing.
提供机构：
Dianheng Wang; Chinese Academy of Medical Sciences & Peking Union Medical College
创建时间：
2023-12-15
