Metabolomic Profiling of CD4+ T cells into Tfh Cells

This dataset comprises metabolomic profiling of CD34 lineage cell differentiation into Tfh cells in transplant arteriosclerosis. Metabolic signatures were analyzed using ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS). Both untargeted and targeted metabolomics approaches were employed to assess metabolic flux in CD4+ T cells at different differentiation stages, with specific focus on mitochondrial one-carbon metabolism. CD4+ T cells were isolated from C57BL/6J background mice lymphoid tissues , and metabolite extraction was performed using 80% methanol, followed by centrifugation and filtration. The LC-MS analysis was conducted in both positive and negative ionization modes, and metabolite identification was performed based on MS1 and MS2 spectra using in-house and public databases. Data processing included peak alignment and normalization using internal standards.