16S rRNA gene sequencing of FMT mice gut microbiota

Mice were subjected to daily oral gavage with antibiotics combination including metronidazole , neomycin sulfate, penicillin , and vancomycin. After a 3-week intervention, the antibiotic-treated group was randomly subdivided into four groups based on body weight and blood lipid levels. Antibiotic interference control group (ABS group): Administered normal saline; PTPS22 administration group after antibiotic interference (ABS_PS group): Intragastrically administered PTPS22 at a dose of 200 mg/kg per day; High-fat diet control flora recipient group (FMT_HFD group): Intragastric administration of fecal bacterial liquid from the HFD group. PTPS22 administered bacteria recipient group (FMT_PS group): Intragastric administration of fecal bacterial liquid from the PTPS22 group. To prepare the fecal bacterial fluid, freshly excreted feces from donor mice were suspended in sterile saline, subjected to centrifugation, and filtered to obtain the fecal bacterial fluid. Each mouse's stomach was then gavage with 0.2 mL of the freshly filtered bacterial solution, and their well-being was observed for a 4-week duration post-gavage.