Immunofluorescent staining of Choline Acetyltransferase (ChAT) in splenocytes from mPGES-1 ko or wt mice treated with LPS

Microscopy images of immunofluorescent staining of choline acetyltransferase (ChAT) in splenocytes from mPGES-1 ko (-/-) or WT (+/+) mice following 20h cultures with or without 10ng/ml LPS. (2 experiments)

Green (L5) = ChAT, Blue (A4) = DAPI, Red (N21) = CD3*

*CD3 staining gave signal in negative control samples so should be disregarded.

The protocol for staining and cell culture is described in the publication "Microsomal
prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry
of the cholinergic anti-inflammatory pathway in endotoxaemic mpuse
spleen" published in PLOS ONE 2017 by the authors: Priya
Revathikumar, Johanna Estelius, Utsa Karmakar, Erwan LeMaître, Marina
Korotkova, Per-Johan Jakobsson and Jon Lampa.