The mechanism research of differentially expressed circRNAs in ALV-J-induced tumor chickens

In this study, we want to screen differentially expressed circRNAs in ALV-J-induced tumor chickens by circRNA-seq, and then conduct the mechanism research of those important circRNAs in ALV-J-induced tumor chickens Overall design: Firstly, one-day old chicks were injected ALV-J, and then cultivated ALV-J-susceptible chickens and ALV-J-resistant chickens. Secondly, at 10 weeks, we collected livers of ALV-J-resistant chickens and ALV-J -susceptible chickens, three livers as three replications were collected from ALV-J -resistant chickens and ALV-J-susceptible chickens. Those collected livers were used for circRNA-seq. Thirdly, analyzed differentially expressed circRNAs in ALV-J-susceptible chickens and ALV-J-resistant chickens. Lastly, research the function of those differentially expressed circRNAs in ALV-J -susceptible chickens which was the model of ALV-J-induced tumor chickens.