Project Achilles

Project Achilles is a systematic effort aimed at identifying and cataloging genetic vulnerabilities across hundreds of genomically characterized cancer cell lines. The project uses genome-wide genetic perturbation reagents (shRNAs or Cas9/sgRNAs) to silence or knock-out individual genes and identify those genes that affect cell survival. Large-scale functional screening of cancer cell lines provides a complementary approach to those studies that aim to characterize the molecular alterations (e.g. mutations, copy number alterations) of primary tumors, such as The Cancer Genome Atlas (TCGA). The overall goal of the project is to identify cancer genetic dependencies and link them to molecular characteristics in order to prioritize targets for therapeutic development and identify the patient population that might benefit from such targets. Project Achilles data is hosted on the Cancer Dependency Map Portal (DepMap) where it has been harmonized with our genomics and cellular models data. You can access the latest and all past datasets here: https://depmap.org/portal/download/all/

阿基里斯计划是一项旨在系统性地识别和编目数百个基因组特征化的癌细胞系中遗传易感性的系统性努力。该项目利用全基因组遗传扰动试剂（如shRNAs或Cas9/sgRNAs）来沉默或敲除单个基因，并识别影响细胞存活的基因。对癌细胞系进行的大规模功能筛选为旨在表征原发肿瘤分子改变（例如突变、拷贝数改变）的研究提供了补充方法，如癌症基因组图谱（TCGA）。项目的总体目标是识别癌症遗传依赖性，并将它们与分子特征相联系，以优先考虑治疗开发的目标，并确定可能从这些目标中获益的患者群体。阿基里斯计划数据托管在癌症依赖性图谱门户（DepMap）上，其中已经与我们的基因组学和细胞模型数据进行了协调。您可以通过以下链接访问最新和所有过往的数据集：https://depmap.org/portal/download/all/

搜集汇总

背景与挑战

背景概述

Project Achilles是一个系统性研究项目，旨在通过全基因组遗传扰动技术识别和分类数百种基因组特征明确的癌细胞系中的遗传脆弱性。该项目数据存储在癌症依赖性图谱门户（DepMap）上，为癌症治疗靶点的优先排序和潜在受益患者群体的识别提供支持。