HPV16 E6 up-regulates MMP-2 and MMP-9 expressions and activities.

Expressions and activities of MMP-2 and MMP-9 in H1299-HPV16E6 induced by PonA were significantly higher than that of parental H1299, H1299-pIND and H1299-HPV16E6 treated with Alc or PonA after 24 hours. (A) RNA was isolated and subjected to RT-PCR analysis using specific primers to MMP-2 and MMP-9. (B) MMP-2 and MMP-9 activities in the culture supernatants were determined by Zymogram. HPV 16 E6-containing SiHa cervical cancer cells were used as positive control (lane 10). The quantitated results for MMP activities in (B) were shown in (C). (D) Levels of MMP-2 and MMP-9 in cell culture mediate was respectively analyzed by MMP-2- and MMP-9- specific ELISA kit (*P<0.05 by t-test).