Genome-wide long non-coding RNAs and mRNAs analysis of the tissues related to ovarian endometriosis

To investigate the function of long non-coding RNA (lncRNA) in ovarian endometriosis and explore its pathogenesis from a new prospective pathway, we conducted genome-wide microarray expression profiling.GO and KEGG analysis demonstrated that the differential mRNAs in ECvsEU and NOvsEU mostly focused on cell adhesion and biological adhesion pathways, nevertheless ECvsNO on molecular metabolism pathways. CHL1 was the most up-regulated mRNA in EC than EU, and the expression of its two antisense lncRNAs, CHL1-AS1 and CHL1-AS2 exhibited the same tendency. They were significantly correlated with each other through bioinformatic calculation.GSEA analysis revealed that CHL1-AS1 and CHL1-AS2 were conspicuously concerned with the same gene set. Validated by qRT-PCR, CHL1-AS2 was higher expressed in EC than EU. It was also up-regulated in NO, and the relative fold changes in EC/EU and NO/EU showed no statistical significance (P>0.05). Furthermore, the value of EC/NO was more closed to 1 when sample size was enlarged, presenting the near expression of CHL1-AS2 in EC and NO. Consequently, we supposed that CHL1, CHL1-AS1 and CHL1-AS2 may play an important part in ovarian endometriosis. We collected ectopic endometrium (EC), normal ovarian (NO) and ectopic endometrium (EU) from four patients with ovarian endometriosis, and compared the lncRNA/mRNA expression files of these three kinds of tissues in pairs