viral metagenome Genome sequencing

The diversity of viruses in herbivorous mammals of the northwest highlands remains largely unexplored.To investigate the viruses of herbivorous mammals in the northwest highlands,A total of 298 samples of seven species of herbivorous mammals were collected from Wuwei and Jinchang, Gansu Province in 2018. The samples were pooled in 36 sample pools depending on the collection target and location. All samples were vigorously vortexed for 5 min.The supernatants were then collected after centrifugation. 500 ul of each supernatant was filtered through a 0.45 um filter to remove eukaryotic and bacterial cell sized particles. The filtrates enriched in viral particles were treated with DNase and RNase to digest unprotected nucleic acid for 60 min. Remaining total nucleic acid was then isolated using QIAamp MinElute Virus Spin Kit according to manufacturer protocol. 36 libraries were then constructed using Nextera XT DNA Sample Preparation Kit and sequenced using the Illumina MiSeq platform with 250 bases paired ends with dual barcoding for each library. For bioinformatics analysis, paired end reads of 250 bp generated by Illumina MiSeq platform were debarcoded using vendor software from Illumina.