Mitofusin 2 Gene Polymorphisms and Metabolic dysfunction Associated Fatty Liver Disease: a case-control study in a Chinese population

A total of 889 subjects presenting at the physical examination center of Third Hospital of Hebei Medical University, Shijiazhuang, China was enrolled in the study. Demographic characteristics including age, gender, height, body weight and biochemical characteristics were collected. Six SNPs (rs2336384, rs873458, rs873457, rs4846085, rs2878677 and rs2236057) were selected based on the previous reports of variants associated with metabolic dysfunction. Rs2336384, rs873458, rs873457 and rs4846085 are located within intron 2; rs2878677 within intron 3 and rs2236057 within intron 11. Genomic DNAs were extracted from peripheral blood leukocytes using a genomic DNA kit (GENEray Biotechnology, Shanghai, China). Mfn2 gene polymorphisms were detected by the ligase detection reaction (LDR). Primer Premier 5.0 software (Premier Biosoft Intl., CA USA) was used to design primers for amplification based on Genbank reference sequence, NG_007945.1. Probes were designed based on GENEray Biotechnology. Genotypes were evaluated using DNA sequencing on an ABI 3730 genetic analyzer (Applied Biosystems, USA), in accordance with the manufacturer’s instructions, and sequenced with GeneMarker V2.2.0 (PA, USA).