Assessment of the effect of the green synthesis process sequence on the antibacterial properties of hybrid Au/TiO₂ nanoparticles supported on a (bio)silica matrix derived from diatoms.

The dataset comprises complete experimental data generated during studies on hybrid Au/TiO₂ nanoparticles synthesized on a (bio)silica matrix derived from diatoms (diatomaceous earth). The primary objective of the research project was to evaluate the effect of the synthesis sequence on the morphology, structure, and antibacterial properties of the resulting nanocomposites. Three distinct synthesis procedures were employed, involving either sequential or simultaneous formation of Au and TiO₂ nanoparticles, using plant extracts from Rosa canina and Ribes nigrum as green reducing agents and biogenic silica obtained from diatomaceous earth as a stabilizing matrix.The dataset includes raw and processed data related to:a) Synthesis proceduresDetailed experimental records documenting reaction conditions, the composition of reagents used, and the course of the three variants of Au/TiO₂/BO composite synthesis.b) Physicochemical characterizationInstrumental data obtained from SEM, TEM, XRD, and XPS analyses. These files contain information on microstructure, morphology, and phase composition, enabling assessment of the influence of the synthesis sequence on nanoparticle growth dynamics, nucleation mechanisms, and interfacial interactions within the composite structures.c) Antibacterial activity studiesResults of minimum inhibitory concentration (MIC) tests conducted in accordance with CLSI guidelines. The dataset includes measurements for various bacterial strains (Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae oraz Staphylococcus aureus), demonstrating differentiated antibacterial efficacy among the individual composites. These data allow evaluation of a potential synergistic effect arising from the simultaneous presence of TiO₂ and Au nanoparticles on a homogeneous silica matrix.