The expression level of microRNA in invasive and nonivasive gonadotrph pituitary tumors

The data include the normalized read counts from smallRNA sequencing of 20 RNA samples from gonadotroph pituitary tumorsThe quality of small RNA fractions was assessed using Agilent 2100 Bioanalyzer with Small RNA Kit Chip (Agilent) and measured with Qubit RNA HS Assay Kits (Thermo Fisher Scientific). One μg of total RNA was used for sequencing library construction with an Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific), according to the manufacturer’s protocol. Ion Xpress™ RNA-Seq Barcode Kit was used for hybridization and ligation of RNA adapters that allows for multiplexed sequencing. RNA reverse transcription and subsequent cDNA purification and library size selection were performed using Nucleic Acid Binding Beads. cDNA was PCR-amplified, followed by DNA purification and size selection. The amount and size distribution of the amplified DNA was determined using Bioanalyzer 2100 using a High Sensitivity DNA Kit (Agilent). The length of miRNA ligation products in barcoded libraries ranged between 94 and114 bp. Template preparation for clonal amplification of up to fourmiRNA libraries at a concentration of 18pM and loading of the PI chip were performed using Ion Chef Instrument, with Ion PI™ Hi-Q™ Chef Kit (Thermo Fisher Scientific). Ion Proton Sequencer (Thermo Fisher Scientific) was used for sequencing. Unmapped bam files were converted into fastq files with a bamToFastq script from bedtools. Read mapping to known human miRNAs (according to miRBase v.22) and reads quantification were performed using miRDeep2.14. Data normalization was performed using DESeq2.