Rice (Oryza stiva L.) nested association mapping project

To explore natural rice variations as a source of useful alleles for agronomic traits improvement in breeding, we generated a NAM population using a Japanese elite cultivar "Hitomebore" as the common parent. Hitomebore was crossed with 20 rice accessions (founders), representing a wide genetic diversity of Oryza sativa. The resulting F1 progeny have been used to generate recombinant inbred lines (RILs) with the single-seed descent method. Finally, we have established 3,000 RILs of F8-F10 generations, with the progeny of each cross represented by 30 - 250 lines.