Distance calculation between Cα atoms of active site residues of WT-ARSA and mutant-ARSA as function of time (100 ns).

Amino acid residues pairs of WT-ARSA active site are Ala10—Asp11, Asp11—Asp12, Asp12—FGL51, FGL51—Arg55, Arg55—Lys105, Lys105—His107, His107—His211, His211—Asp263, Asp263—Asn264, Asn264—Lys284, Lys284—Ala10 and those of mutant-ARSA are Ala10—Asp11, Asp11—Asp12, Asp12—FGL57, FGL57—Arg61, Arg61—Lys111, Lys111—His113, His113—His217, His217—Asp269, Asp269—Asn270, Asn270—Lys290, Lys290—Ala10. (ZIP)