Transcriptome level regulations for desiccation and submergence tolerance in intertidal seaweed species Ulva lactuca. Ulva lactuca

This study aimed at identification of genetic regulations for desiccation and submergence tolerance in intertidal seaweed species Ulva lactuca which arisies periodically due to tidal rhythms. Overall design: The algal thalli were collected and acclamatised to laboratory. The experiments were designed for desiccation for different durations at a moisture condition of 20%. The thalli were desiccated for 24 h, 36 h and 72 h and investigated for transcriptomics by RNA-seq. After each desiccation time point, the thalli were resubmerged in autoclaved seawater. After 1 h of resubmergence, thalli were collected and processed for RNA extraction and subsequent RNA-seq. The transtion of desiccation to submergence will determine the genetic regulations for submergence tolerance in this group of plants.