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Single cell gene expression profiling of cardiac non-myocytes from CD31 depleted suspensions

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Cardiac non-myocytes are poorly defined as a cell type. In order to better define them, we performed single cell RNA sequencing to assess heterogeneity. Non-myocytes were isolated by mincing murine cardiac tissue and then performing magnetic bead removal of enodthelial cells to enrich minor non-myocyte populations.

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