Chinese Herbal Formula (GCNY)-Medicated Serum Alleviates Peroxidation Induced by H2O2 in Human Microglial Cells

Transitional Chinese herbal medicine has a long history of applications for anti-aging and longevity purposes, and in our patent formula Gancao Nurish-Yin (GCNY) decoction, all of the ingredients show antioxidant properties. However, in real clinical practice, extractions of herbs are rarely applied alone but are prescribed as integrated formula. In order to investigate whether GCNY possess anti-oxidation potential, we applied GCNY as a formula to treat rats in order to acquire medicated serum, which was then added on H2O2 (200 μM)-modeled human microglial cell line HMC-3 in comparison with its controls. To explore the impact of H2O2 modeling to HMC-3 cells and how GCNY-medicated serum affects transcriptome of H2O2 modeled cells, high-throughput RNA sequencing analysis was performed in NC, NC+H2O2 and GCNY groups of cells.