The ankle–brachial index, gastrocnemius mitochondrial respirometry, and walking performance in people with and without peripheral artery disease

Background: Mitochondrial abnormalities exist in lower-extremity peripheral artery disease (PAD), yet the association of the ankle–brachial index (ABI) with mitochondrial respiration in gastrocnemius muscle is unknown. The association of gastrocnemius mitochondrial respiration with 6-minute walk distance in PAD is unknown. This objective of this study was to describe associations of the ABI with mitochondrial respiratory function in gastrocnemius muscle biopsies and associations of gastrocnemius mitochondrial respirometry with 6-minute walk distance in people with and without PAD. Methods: People with (ABI ⩽ 0.90) and without (ABI 1.00–1.40) PAD were enrolled. ABI and 6-minute walk distance were measured. Mitochondrial function of permeabilized myofibers from gastrocnemius biopsies was measured with high-resolution respirometry. Results: A total of 30 people with PAD (71.7 years, mean ABI: 0.64) and 68 without PAD (71.8 years, ABI: 1.17) participated. In non-PAD participants, higher ABI..., , # **The ankle–brachial index, gastrocnemius mitochondrial respirometry, and walking performance in people with and without peripheral artery disease**

Dataset DOI: [10.5061/dryad.xksn02vtj](10.5061/dryad.xksn02vtj)

**Description of the data and file structure**

Participants with and without PAD were enrolled from the Chicago, Illinois area. The study was approved by a university Institutional Review Board. All participants provided written informed consent.

Participants with and without PAD were identified using lists of patients and by advertisements placed on Chicago Transit Authority buses and trains, and by mailed recruitment postcards, using a purchased mailing list. Enrollment occurred between 7/23/2018 and 8/26/2021.

PAD was defined as an ABI < 0.90 in either leg (914). Absence of PAD was defined as an ABI of 1.00-1.40. Potential participants with ABI of 0.90 to 0.99 were excluded, because they may have mild lower extremity atherosclerosis. To attain similar ages between pe..., This file contains de-identified data from this study which looked at mitochondrial function in people with and without peripheral artery disease. Only de-identified data is available here.