A new mixed-ligand coordination polymer: crystal structure and protective effect on viral myocarditis via regulating inflammatory response <i>in vivo</i>

A new Co(II)-based coordination polymer {[Co(ttpa)(1,4-bdc)]·H₂O}<i>_n</i> (<b>1</b>) as well as its nanoparticles (denoted as nano <b>1</b> hereafter) have been synthesized using hydrothermal and hand grinding methods (ttpa = tris(4-(1,2,4-triazol-1-yl)phenyl)amine, 1,4-bdc = 1,4-benzenedicarboxylate). Furthermore, the protective effect of nano <b>1</b> on acute viral myocarditis mice was evaluated. Firstly, the CVB3-induced acute viral myocarditis model was successfully constructed and then nano <b>1</b> was given for indicated treatment. The <i>in vivo</i> activity of nano <b>1</b> was evaluated with mice survival rate and the CVB3 titer determination. The level of the inflammatory cytokines in the mice after treatment was measured with enzyme linked immunosorbent assay (ELISA) detection kit.本研究采用水热法与手工研磨法，合成了一种新型二价钴基配位聚合物{[Co(ttpa)(1,4-bdc)]·H₂O}_n（记为化合物1）及其纳米颗粒（下文简称纳米1），其中ttpa为三(4-(1,2,4-三唑-1-基)苯基)胺，1,4-bdc为1,4-苯二甲酸根。进一步评估了纳米1对急性病毒性心肌炎小鼠的保护作用：首先成功构建柯萨奇病毒B3（CVB3）诱导的急性病毒性心肌炎小鼠模型，随后给予纳米1进行指定治疗；通过小鼠存活率与CVB3滴度检测，评价纳米1的体内活性；采用酶联免疫吸附测定（ELISA）试剂盒，测定给药后小鼠体内炎症细胞因子的水平。