Hantavirus Amplicon-based NGS Approaches

This work describes whole-genome sequencing of New World and Old World hantavirus species for the purpose of identifying optimal sequencing approaches for research, environmental or clinical specimens to be used for a variety of applications. We designed a 500bp-1kb non-overlapping amplicon tiling approach and implemented a variety of primer pooling methods and PCR amplifications to understand SNP incorporation between each method. We examined these approaches on the Illumina MiSeq and Oxford Nanopore Technologies MinION device.