Transcription profiling of mouse skin from epithelial activated beta-catenin mutant embryo

Beta-catenin signaling is required for hair follicle development, but it is unknown whether it is sufficient to activate expression of hair follicle genes in embryonic skin. To address this we profiled gene expression in skin dissected from E14.5 KRT14-Cre Ctnnb1(Ex3)fl/+ embryos carrying an activating mutation in epithelial beta-catenin, and control littermate embryos. Experiment Overall Design: Total skin RNA from two KRT14-Cre Ctnnb1(Ex3)fl/+ and two control littermate E14.5 embryos was hybridized to Affymetrix GeneChip Mouse Genome MOE430 2.0 oligonucleotide microarrays. Experiment Overall Design: Appended below is Table S3: Full list of differentially expressed genes in KRT14-Cre Ctnnb1(Ex3)fl/+ mutant compared with control littermate intact skin at E14.5, including normalization and filter parameters. Fold change, listed in the second column, gives the ratio of normalized mutant : control transcript levels.β-连环蛋白信号通路（beta-catenin signaling）是毛囊发育所必需的，但目前尚不明确其是否足以激活胚胎皮肤中毛囊相关基因的表达。为解答这一科学问题，我们对携带上皮细胞β-连环蛋白（beta-catenin）激活突变的E14.5龄KRT14-Cre Ctnnb1(Ex3)fl/+胚胎，以及同窝对照胚胎的皮肤组织开展了基因表达谱分析。 实验总体设计：提取2只KRT14-Cre Ctnnb1(Ex3)fl/+突变胚胎与2只同窝对照胚胎的总皮肤RNA，将其与Affymetrix GeneChip小鼠基因组MOE430 2.0寡核苷酸微阵列进行杂交。 实验总体设计：附随的表S3完整列出了E14.5龄KRT14-Cre Ctnnb1(Ex3)fl/+突变胚胎与同窝对照完整皮肤组织间的差异表达基因全集，其中包含标准化处理与筛选参数。第二列所示的倍数变化（Fold change）为标准化后的突变组与对照组转录本水平的比值。