Identification of milk proteins using mass-spectrometry.

The number of amino acids (aa) and the expected molecular weight (MW) are given for the mature proteins (i.e. without the signal peptide) where appropriate. In addition, the reported molecular weights for the mouse caseins [28] and the molecular weights observed in the SDS-PAGE analysis are shown. The MS score is a measure of confidence of the detected protein species. A score higher than 39 is significant. The number of peptide sequences and the fraction of the total protein covered are also indicated. Protein bands #1-8 are the predominant protein species in the milk of wild-type mice. Protein bands #9-13 are detected in the milk of α-casein deficient mice but not in the milk of wild-type or heterozygous mice. L-AAO-1: L-amino acid-oxidase1, PDIA6: protein disulfide isomerase associated 6.