A pyruvate-centered metabolic regulation mechanism in Escherichia coli

In this study, a general regulated mechanism of prpD and malK has been firstly revealed through transcriptome analysis and many experimental verifications. We proved that overexpression of malK could up-regulated the expression of prpD and propanoate metabolism which is the main factor for the increasing expression of exogenous genes. More importantly, the overexpression of prpD or malK could arouse a complex set of pyruvate-centered metabolic networks which mainly increase the energy supplying (ATP), by-products recycling (acetate), and amino acids for the efficient expression of exogenous genes.