Differential 3’ Processing of Specific Transcripts Expands Regulatory and Protein Diversity Across Neuronal Cell Types

Alternative polyadenylation (APA) regulates mRNA translation, stability, and protein localization. However, it is unclear to what extent APA regulates these processes uniquely in specific cell types. Using a new technique, cTag-PAPERCLIP, we discovered significant differences in APA between the principal types of mouse cerebellar neurons, the Purkinje and granule cells, as well as between proliferating and differentiated granule cells. Transcripts that differed in APA in these comparisons were enriched in key neuronal functions and many differed in coding sequence in addition to 3’UTR length. Examination of Pabpc1-GFP bound RNA in cerebellar granule in Purkinje cells and cerebellar granule cells during development using a cTag-PAPERCLIP. RNA-seq of RNA from cerebellar granule cells at two developmental timepoints to compare with cTag-PAPERCLIP data.