miRNA expression in the extracellular vescle (EV) fraction

To developed the mothod for obtaining miRNA information in cells non-destructively, we aimed to produce EVs encapsulating miRNA-binding protein fragments that actively incorporate miRNAs into EVs. We then performed gene expression profiling analysis using data obtained from RNA-seq of control cells and cells co-expressing EV-inducing protein and miRNA binding protein. Gene expression profiling analysis of RNA-seq data for HEK293T control cells and cells co-transfected with the EV-inducing protein (EPN-01) and the miRNA binding protein fragment (Flag-MID-PIWI-Vpr