T cell characteristics associated with toxicity to immune checkpoint blockade in patients with melanoma [scRNA-Seq]. T cell characteristics associated with toxicity to immune checkpoint blockade in patients with melanoma [scRNA-Seq]

Severe immune-related adverse events (irAEs) occur in up to 60% of melanoma patients treated with immune checkpoint inhibitors (ICIs). However, it remains unknown whether a common baseline immunological state precedes irAE development. Leveraging CyTOF, single-cell RNA sequencing, bulk RNA sequencing, and T cell receptor sequencing to analyze pretreatment blood from metastatic melanoma patients treated with ICIs, we investigated cellular factors associated with severe irAE development regardless of organ system involvement. Our results demonstrate circulating T cell characteristics associated with ICI-induced toxicity, with implications for improved diagnostics and clinical management. Overall design: To investigate cellular determinants of severe irAE development, we performed single-cell RNA sequencing (scRNA-seq) on peripheral blood mononuclear cells (PBMCs) obtained pretreatment from patients with metastatic melanoma treated with immune checkpoint blockade. ---------------------------------------- Authors state "raw data are not available due to patient privacy concerns".