Combined RNA-seq and RAT-seq mapping of long noncoding RNAs in pluripotent reprogramming

RNA transcriptome sequencing (RNA-Seq) and RNA reverse transcription-associated trap sequencing (RAT-Seq) were usded to map long noncoding RNA (lncRNAs) in reprogramming of fibroblasts into pluripotency Overall design: Fibroblasts (FIB) and induced pluripotent stem cells (iPSCs) were collected in the process of reprogramming. RNA-Seq was used to identify lncRNAs that are differentially-expressed in reprogramming. RAT-Seq was utilized to generate the database to map the regulatory element network of lncRNA candidate Osclr8 (NONMMUT043505, Platr10).