Interlaboratory evaluation of LC–MS-basedbiomarker assays: supplementary materials

Validation of biomarker assays is crucial for effective drug development and clinical applications.
Interlaboratory reproducibility is vital for reliable comparison and combination of data from different
centers. This review summarizes interlaboratory studies of quantitative LC–MS-based biomarker assays
using reference standards for calibration curves. The following points are discussed: trends in reports,
reference and internal standards, evaluation of analytical validation parameters, study sample analysis
and normalization of biomarker assay data. Full evaluation of these parameters in interlaboratory studies
is limited, necessitating further research. Some reports suggest methods to address variations in biomarker
assay data among laboratories, facilitating organized studies and data combination. Method validation
across laboratories is crucial for reducing interlaboratory differences and reflecting target biomarker
responses.