Tested AN11251 and doxycycline combination treatments provide no improvement on Wolbachia reduction in comparison to the monotherapies.

Shown are number of female worms with less or more than 99% of Wolbachia reduction as well as the frequency of female worms, which presented a Wolbachia reduction greater than 99%. Female adult worms were isolated from wild-type BALB/c mice that had been infected for 35 days with L. sigmodontis and treated with different AN11251 (50, 100 and 200 mg/kg) or doxycycline (40 mg/kg) concentrations alone or in combination for 7 and 14 days. Drugs were given as a twice-daily oral dosage (BID). N = 5.