Occurrence chart of all long-read and V4 region ASVs per specimen and their read abundances.

There are individual sheets for Collodaria, Nassellaria, and Spumellaria specimens, titled either “full-length ASVs” or “V4-only” respectively. The first column of each sheet lists the ASV identifiers (as discussed in the text and displayed in figures). The second column gives the full ASV sequence. The third column, “Taxon,” provides the taxonomic assignment of each ASV based on PR2 reference taxonomy, and the “Confidence” column lists the confidence level of each molecular taxonomic assignment on a scale from 0–1. Subsequent columns give the specimen morphological taxonomic assignments with numeric identifiers (as referenced throughout in the manuscript text and in figures), and the absolute number of reads of each ASV produced by individual specimens. All ASVs that matched the family assignment of each morpho-specimen are included, even those with <3 reads per specimen. The sheets containing extracted V4-regions of each ASV indicate which of the original long-read sequences had unique V4 regions versus identical V4 matches with other sequences (causing their read counts to be merged in the occurrence chart). Each merged V4 sequence is labelled “merged” in the ASV_ID column; all other V4 ASVs retain the same IDs as their long-read counterparts. For the analyses in this study, ASVs with <3 reads in each specimen were removed to account for potential contamination (see “Materials and methods”), but the data in raw form is provided here to enable maximum flexibility in future use.
(XLSX)