KSM fits for 320 bait-prey pairs of NUPs in KARMA assays

Supplementary analyzed data from Manuscript: TITLE: Maturation kinetics of a multiprotein complex revealed by metabolic labeling. JOURNAL: CELL. Article Type: Research Article Authors: Evgeny Onischenko*, Elad Noor*, Jonas S. Fischer*, Ludovic Gillet, Matthias Wojtynek, Pascal Vallotton, Karsten Weis *Equally Contributing Authors Corresponding Authors: Evgeny Onischenko and Karsten Weis Related to Figures S4, Figure S5, and Table S3; KSM fitting curves for 320 bait-prey pairs characterised in the KARMA assays. Individual plots: The red line represents the expected labeling of the reference proteins as a function of time, and is used to find the effective sampling time for each sample (i.e. the x-value of each point). Then, we try to fit the 3-step KSM (purple line). Preys with metabolic labeling exceeding the reference protein were fitted with a 4-step model. 𝜙1: effective maturation pool, 𝜙2: accessible fraction of the mature state.本数据集为对应手稿的补充分析数据：手稿标题为《通过代谢标记揭示多蛋白复合物的成熟动力学》，发表期刊为《CELL》，文章类型为研究论文。作者包括：Evgeny Onischenko*、Elad Noor*、Jonas S. Fischer*、Ludovic Gillet、Matthias Wojtynek、Pascal Vallotton、Karsten Weis；*标注作者为共同第一作者，通讯作者为Evgeny Onischenko与Karsten Weis。本补充数据对应图S4、图S5及表S3，包含KARMA实验中320组诱饵-猎物对的KSM拟合曲线。单幅绘图说明如下：红色曲线代表参考蛋白质的标记水平随时间的变化关系，用于确定每个样本的有效采样时间（即各数据点的横坐标值）；随后我们尝试采用3步KSM模型进行拟合（紫色曲线）；对于代谢标记水平高于参考蛋白的猎物蛋白，则采用4步模型进行拟合。其中，𝜙1代表有效成熟池，𝜙2代表成熟状态的可及组分。