RTK_NFPA.xlsx

Non-functioning pituitary adenomas (NFPA) account for approximately one third of all pituitary adenomas. These tumors may be locally invasive and also recurrent. Markers of recurrence are needed to guide patient management. Recteptor Tyrosina Kinases (RTK) may sereve as prognostic and therapeutic marker. To examine differential RTK phosphorylation pattern of recurrent NFPAs, we recruited 20 patients and grouped them as non-recurrent (n=10), and recurrent (n=10).Follow-up of 15 years was used to determine the recurrence. We then performed a membrane-based antibody array (ARY001B) for the parallel determination of the relative phosphorylation of 49 tyrosine kinases in recurrent NFPAs. As the experiment was replicated on two set of membranes, each tyrosine kinase was represented in quadruples. Student’s t-test was performed to compare the mean between two groups.