RNA polymerase evolution FASTQ files (2/2)

RNA polymerase (RNAP) is emblematic of complex biological systems that control multiple traits involving trade-offs such as growth versus maintenance. Laboratory evolution has revealed that mutations in RNAP subunits, including RpoB, are frequently selected. However, we lack a systems view of how mutations alter the RNAP molecular functions to promote adaptation. We, therefore, measured the fitness of thousands of rpoB variants under multiple conditions and genetic backgrounds, to find that adaptive mutations cluster in two separate modules. Mutations in one module favor growth over maintenance through a partial loss of an interaction associated with faster elongation. Mutations in the other favor maintenance over growth through a destabilized RNAP-DNA complex. The two molecular handles capture the versatile RNAP-mediated adaptations. Combining both interaction losses simultaneously improved maintenance and growth, challenging the idea that growth-maintenance tradeoff resorts only from ..., , , # Title of Dataset

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The dataset is linked to the above article. Here we have uploaded the sequencing data. The code and processed data can be found in linked Dryad: DOI: 10.5061/dryad.n8pk0p30n

## Description of the data and file structure

The information about the fastq data and the associated samples from which they were extracted can be found in the tsv file: Sample checklist\_1674571220193.tsv. Details on the file experimental sample map can be found in the tsv file: fastq2\_template\_1674570722019.tsv. The files are in the dryad submission: 10.5061/dryad.n8pk0p30n

See that submission for more details on this data.

## Code/Software

The detailed code can be found at:https://github.com/Alaksh/RNA-Polymerase-Evolution.git and Zenodo link: 10.5281/zenodo.8144064