Primary capillarized LSEC isolated from liver fibrotic mouse injected with UCMSC

Re-differentiation of capillarized LSEC is promising to resolute liver fibrosis. We describe that UCMSC is able to initiate LSEC differentiation. We profiled major changes UCMSC brought to LSEC in liver fibrotic mice. miR-325 is responsible for LSEC differentiation and associated fibrosis resolution by initiating a very special structure, multiple sieve plate areas without observable fenestrations. The specialized LSEC inhibited activated HSC and F480 positive macrophage to level of WT control. Analysis of proteome changes in LSEC revealed networks of genes, especially PTPRM and YWHAQ in LSEC specialized differentiation, with similar trends in liver fibrosis patients, offering potential therapeutic targets.