The 16srRNA data of EcN C6 on gut microbes in the hyperuricemia rat model.

Feces collected from hyperuricemia rats were frozen in -80 ℃ until use. DNA was extracted using the E.Z.N.A. Stool DNA Kit (OMEGA, USA) following the instructions. 16S rDNA (V4 region) was amplified by a two-step PCR enrichment using barcodes for multiplexing. The pooled DNA was cleaned utilizing Ampure XP beads (Beckman, USA). DNA libraries were constructed using NEBNext Ultra II FS DNA Library Prep kit (NEB, USA) and were sequenced by Illumina HiSeq X Ten platform.

从高尿酸血症大鼠中收集的粪便样本在-80℃下冷冻保存，直至使用。采用E.Z.N.A.粪便DNA提取试剂盒（OMEGA，美国）按照说明书进行DNA提取。通过两步PCR扩增法对16S rDNA（V4区域）进行富集，并使用条形码进行多重测序。利用Ampure XP磁珠（Beckman，美国）对混合DNA进行纯化。使用NEBNext Ultra II FS DNA文库构建试剂盒（NEB，美国）构建DNA文库，并通过Illumina HiSeq X Ten平台进行测序。