Cellular dynamics of pig-to-human kdiney xenotransplantation [RNA-seq]

We performed comprehensive single-cell RNA sequencing (scRNA-seq) analyses of the two porcine xenografts and their contralateral untransplanted kidneys to dissect xenotransplantation-associated cellular dynamics and xenograft-recipient interactions. We additionally performed longitudinal bulk RNA-seq on core biopsies to dissect the time-resolved physiological changes of the xenografts and longitudinal scRNA-seq of peripheral blood mononuclear cells (PBMCs) to detect recipient’s immune responses across time. Longitudinal biopsy samples were collected in xenotransplantation case 2 at 0h from untransplanted control kidney, and then at 12h, 24h, and 48h from the xenograft.