Single-cell and bulk RNA sequencing of mouse atherosclerosis disease stage course [scRNASeq_WAT_Mono]

Atherosclerotic coronary artery disease (CAD) development encompasses changes in immune cell activation across different tissues, including white adipose tissue (WAT). However, the specific disease-associated transcriptional changes occurring within each of the tissue cell types remain incompletely characterized. Here, we provide a scRNA-seq dataset allowing the characterization of disease-associated cell states within the major WAT cell types. For the myeloid cells, we further analyzed sub-populations and characterized their protein-coding and miRNA gene markers. By comparing to naive monocytes isolated from blood, we further used the tissue profiles to interrogate how the tissue infiltration changes the monocyte and dendritic cell transcriptome profiles. A disease stage course of atherosclerosis in mouse was established by varying genotype (wild type and proatherogenic) and diet (0, 1 or 3 months of high-fat diet), followed by scRNA-Seq (all cell types) and bulk RNA-Seq of the thoracic aorta.