Genome-wide maps of RNA polymerase II occupancy in immortalized MEF cells [ChIP-seq]

We report the application of ChIP-seq using an anti-RNA pol II antibody to determine changes in RNAP II occupancy patterns genome-wide. We found that as a result of MYC expression, RNAP II levels increased at both the promoter and gene body regions. This suggests that MYC increases the amount of both the initiation and elongation steps of trasncription globally. Examination of RNA polymerase II genome occupancy comparing the effect of the absence of Myc to two full length versions of Myc