Rewilding shows differential gene expression in sympatric Physella acuta (Draparnaud, 1805) snail lineages

This dataset contains 3,302 differentially expressed genes (DEGs) from the freshwater snail Physella acuta, identified through transcriptomic analysis comparing environmental conditions (Field vs. Lab) across both invasive (A) and native (B) lineages. The FASTA file includes sequences for both upregulated (URGs) and downregulated genes (DRGs) from four pairwise comparisons: Field A vs. Field B, Field B vs. Lab B, Field A vs. Lab A, and Lab A vs. Lab B. DEGs were consistently identified using two independent RNA-seq analysis pipelines—STAR/Limma and Kallisto/DESeq2—ensuring robustness of detection. Additionally, this dataset includes a subset of 1,228 transcript sequences representing orthologs of human and mouse MitoCarta genes. The MitoCarta database catalogs nuclear-encoded mitochondrial proteins involved in ATP synthesis, metabolism, and cellular stress responses. These sequences were identified through transcriptome-wide similarity searches against the MitoCarta 3.0 reference set (R..., , # Rewilding shows differential gene expression in sympatric Physella acuta (Draparnaud, 1805) snail lineages

Dataset DOI: [10.5061/dryad.wstqjq2z8](10.5061/dryad.wstqjq2z8)

## Description of the data and file structure

Laboratory-bred *Physella acuta* snails from two genetically distinct lineages (A and B) were exposed to either controlled laboratory or natural field conditions for one week to investigate molecular mechanisms underlying differential fitness. RNA-seq was performed on these snails, and differential gene expression was analyzed using STAR/Limma and Kallisto/DESeq2. The resulting dataset includes 3,302 differentially expressed genes and a set of 1,228 transcript sequences identified as orthologs of vertebrate mitochondrial genes based on similarity to the MitoCarta 3.0 database. Transcriptome assembly was genome-guided and incorporated RNA-seq data from both rewilded and laboratory conditions.

### Files and variables

#### File: 3302\_ALL\_Physella\_acuta\_DEGS.fasta

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