Structural and functional significance of two conserved lysine residues in acylated sites of Kingella kingae RtxA cytotoxin
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https://zenodo.org/record/14870820
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资源简介：
Data underlying the figures in the publication “Structural and functional significance of two conserved lysine residues in acylated sites of Kingella kingae RtxA cytotoxin”, published in Biochimie, 2024 Dec 31:S0300-9084(24)00320-1.
https://doi.org/10.1016/j.biochi.2024.12.016
Description of Files:
THS_2A: Functional complementation of four different fusion pairs, quantified by measuring β-galactosidase activity in bacterial extracts of E. coli strain BTH101.
THS_2B: Functional complementation of T25-RtxC with T18-proRtxA529–718 fusion variants.
THS_2C: Functional complementation of T25-RtxC with T18-proRtxA529–623, T18-proRtxA624–718, or T18-proRtxA529–718.
THS_2D: Functional complementation of T25-RtxC with T18-proRtxA529–623 fusion variants containing substitutions K558R and K558Q.
THS_2E: Functional complementation of T25-RtxC with the T18-proRtxA624–718 fusion variants containing substitutions K689R and K689Q.
Binding_3BL: Cell binding of RtxA variants to sheep erythrocytes.
Binding_3BR: Cell binding of RtxA variants to sheep erythrocytes after washing with alkaline carbonate.
Far-UV_3CL: Far-UV spectra of RtxA variants.
Near-UV_3CR: Near-UV spectra of RtxA variants.
Lysis_4A: Erythrocyte lysis in the presence of RtxA variants.
创建时间：
2025-02-14
