Residual feed intake in peripartal dairy cows is associated with differences in milk fat yield, ruminal bacteria, biopolymer hydrolyzing enzymes, and circulating biomarkers of immunometabolism

Supplemental Table S1. Ingredient composition of TMR diets fed during close-up (-28 d to calving) and fresh (from 1 to 28 DIM) periods in the 5 different published studies used for residual feed intake (RFI) evaluation. Supplemental Table S2. Nutrient composition and energy density (NRC, 2001) of TMR diets fed during close-up (-28 d to calving) and fresh (from 1 to 28 DIM) periods in the 5 different published studies used for residual feed intake (RFI) evaluation. Supplemental Table S3. Species-specific primers for the quantification of 17 key ruminal bacteria by quantitative RT-PCR assay in least-efficient and most-efficient multiparous Holstein dairy cows during prepartal and early lactation periods. Supplemental Table S4. Milk yield and composition in least-efficient (L-eff) and most-efficient (M-eff) multiparous Holstein dairy cows during the first 30 d of lactation in the subset used for blood plasma biomarker analyses. Supplemental Table S5. Milk yield and composition in least-efficient (L-eff) and most-efficient (M-eff) multiparous Holstein dairy cows during the first 30 d of lactation in the subset used for ruminal digesta sampling for measuring biopolymer degrading enzyme activities.

补充表 S1：在用于残余采食量（RFI）评估的5项不同已发表研究中，近产期（-28天至分娩）及新产期（从第1天至第28天干乳期）喂食的TMR饲料成分。补充表 S2：在用于残余采食量（RFI）评估的5项不同已发表研究中，近产期（-28天至分娩）及新产期（从第1天至第28天干乳期）喂食的TMR饲料的营养成分组成及能量密度（NRC，2001）。补充表 S3：针对最不高效和多效的经产荷斯坦奶牛，在产前及早期泌乳期，通过定量RT-PCR检测方法量化17种关键瘤胃细菌的物种特异性引物。补充表 S4：在用于血液血浆生物标志物分析的子集中，最不高效（L-eff）和多效（M-eff）经产荷斯坦奶牛在泌乳初期30天内的产奶量及成分。补充表 S5：在用于测量生物聚合物降解酶活性的瘤胃消化物采样子集中，最不高效（L-eff）和多效（M-eff）经产荷斯坦奶牛在泌乳初期30天内的产奶量及成分。