Small RNA-seq analysis of E10.5 Tbx18-expressing peri-wolffian duct stroma

The overall goal of this project is to characterize the repertoire of miRNAs in E10.5 Tbx18-expressing peri-wolffian duct stroma cells during development. To achieve this, E10.5 peri-wolffian ducts were dissected from timed-mated Tbx18-Cre;CAG-tdTomato embryos, followed by FACS sorting based on RFP expression to enrich for Tbx18-expressing cells. Overall design: 1X75 bp standard RNA-seq was performed using an Illumina NextSeq platform.