CCDB_8063.jpg

Processed mosaic of a sagittal section through the brain of mouse model
of glioma. To produce transformed cells, creGFAP mice were injected in
the cortex with Ras/s.i.p53 virus and perfused after 5 weeks. In this
system, GFP is introduced by the virus and expression is induced by an
IRES site in the mRNA. Thus, the GFP gene is expressed whenever the
virus infects a cell expressing GFAP (green). The section was
immunolabeled for nestin (red) and counterstained with DAPI (blue). See
MP:7857 for a section from the same brain labeled with GFAP.

经过处理的脑组织切片镶嵌图，切片来自胶质瘤小鼠模型。为产生转化细胞，通过向皮质注射 Ras/s.i.p53 病毒对 creGFAP 小鼠进行接种，并在五周后进行灌注。在此系统中，GFP 由病毒引入，并通过 mRNA 中的 IRES 位点诱导表达。因此，当病毒感染表达 GFAP（绿色）的细胞时，GFP 基因便会表达。切片经 nestin（红色）免疫标记并使用 DAPI（蓝色）进行复染。参见 MP:7857 以获取同一脑部切片，该切片经 GFAP 标记。