Amentoflavone induces cell autophagy through promoting phosphorylation of ULK1 in hepatocellular carcinoma

Purpose: To date, the biological activity of AMF has not been fully investigated. We set out to analyze how AMF regulates gene expression in HepG2 cells. Conclusions: we performed clustering analysis on the various expressed genes associated with autophagy, and the pathways confirmed in the KEGG and BP analysis Methods: RNA-seq was performed with two repetitions in HepG2 followed by treatment with DMSO and 80 μM Tub B for 48 h.