Botrytis cinerea proteomic data infecting Phaseolus vulgaris at 48 hours post infection

The project aims to identify secreted proteins from Botrytis cinerea during the interaction of Phaseolus vulgaris leaves. Sample and data processing protocols:Botrytis cinerea conidial suspensions were inoculated onto Phaseolus vulgaris leaves and incubated in a humid chamber under a 12 h light/12 h dark photoperiod at 20°C. Infection droplets were collected at 48 h post-inoculation (hpi), and residual fungal material was removed using a 0.22 μm PVDF syringe filter. Proteins were extracted in lysis buffer containing detergents, reducing agents, and protease inhibitors. Protein extracts were reduced, alkylated, and digested with sequencing-grade trypsin prior to LC-MS/MS analysis. Tryptic peptides were analyzed using an Evosep One LC system coupled to a Bruker timsTOF Pro mass spectrometer operating in PASEF mode. Peptide separation was performed on a 15 cm × 150 μm C18 column using a 2–35% acetonitrile gradient containing 0.1% formic acid. MS data were acquired over an m/z range of 100–1700 using 10 PASEF scans per cycle.