Sputum_analysis_NaviosEX_cytometer

To determine whether our method for sputum processing can be applied to different flow cytometric platforms.
Conclusion:
Using our methods, we were able to successfully dissociate sputum into a single cell suspension into live, viable cells that were stained with blood and epithelial lineage markers and analyzed by three flow cytometric platforms.
Notes:
Three-day sputum collection from persons aged 55-74 with at least a 30 pack-year smoking history at high risk of developing lung cancer. This experiment was used as a proof of concept of our protocol for a methods manuscript. No treatments or experimental conditions were applied to the samples. FlowCheck was performed to check the fluidics and optics. FlowSet was performed to standardize the machine for the sputum specific settings. The presence of alveolar macrophages was verified to determine the sample derives from the lung.